منابع مشابه
Phagolysosomal pH in alveolar macrophages.
We studied phagolysosomal pH in alveolar macrophages (AM) using fluorescein-labeled yeast (FYP) and silica particles (FSP) as probes. Fluorescence intensities from the ingested test particles were measured on populations of AM using fluorescence spectrometry and on individual phagolysosomes using fluorescence microscopy. Measurements were performed on rabbit AM, which had been incubated with FY...
متن کاملPhagolysosomal pH of human neutrophils.
Human neutrophils sequester yeast cells or zymosan particles in two classes of phagocytic vacuoles: sealed and unsealed. The pH of sealed vacuoles was measured by a newly devised fluorometric procedure that used fluoresceinated zymosan particles, derived from Saccharomyces cerevisiae, as a pH probe. Five minutes after initiation of phagocytosis, sealed vacuoles were alkaline, exhibiting a pH of...
متن کاملInhibition of Phagolysosomal Biogenesis by the Leishmania Lipophosphoglycan
Whereas amastigotes of the protozoan parasite Leishmania proliferate inside acidic phagolysosomal vacuoles of the macrophage, vacuoles induced by Leishmania donovani promastigotes during initiation of infection are poorly characterized. Here, evidence is presented that interaction of these parasitophorous vacuoles with endocytic organelles is very limited. In contrast, vacuoles formed around L....
متن کاملhuman trafficking in central asia
human trafficking is one form of organized crime with 7 to 10 billion dollars profit next to drug and weapon trades. former soviet republics and eastern europe have become the newest sources of human trafficking, for europe and north america for the purpose of sexual exploitation .this article is a result in causes of human trafficking in central asian republic of the former soviet :union:.
متن کاملHuman dendritic cell activity against Histoplasma capsulatum is mediated via phagolysosomal fusion.
Histoplasma capsulatum is a fungal pathogen that requires the induction of cell-mediated immunity (CMI) for host survival. We have demonstrated that human dendritic cells (DC) phagocytose H. capsulatum yeasts and, unlike human macrophages (Mø) that are permissive for intracellular growth, DC killed and degraded the fungus. In the present study, we sought to determine whether the mechanism(s) by...
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ژورنال
عنوان ژورنال: BIO-PROTOCOL
سال: 2014
ISSN: 2331-8325
DOI: 10.21769/bioprotoc.1163